qPCR NEWS - May 2012 - focus on MIQE guidelines
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Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and RT-qPCR), which are compiled and summarised on the
Gene Quantification homepage. The focus of this newsletter issue is:

* Update on the MIQE pages  -   http://www.***.com/
* New updated APP versions available in May 2012 for iOS, Android, and html5  -   http://www.***.com/
* Follow ou new established "GeneQuan Daily NEWS" - a daily updated newsletter  -   http://www.***.com/
* GenEx - a powerful tool For qPCR data analysis - download a free trial version  -   http://www.***.com/

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The MIQE Guidelines - Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Stephen A. Bustin,  Vladimir Benes,  Jeremy A. Garson,  Jan Hellemans,  Jim Huggett, Mikael Kubista,  Reinhold Mueller,  Tania Nolan,  Michael W. Pfaffl,  Gregory L. Shipley, Jo Vandesompele,  and  Carl T. Wittwer
Clinical Chemistry 2009, 55(4): 611-622
http://www.***.com/

BACKGROUND:  Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments.
CONTENT:  The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.
SUMMARY:  Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.
http://www.***.com/

The MIQE guidelines has been frequently cited by other researchers:  => 754  times until May 2012
=> http://www.***.com/

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updated APP versions for iOS, Android and html5

MIQE qPCR APP for iOS -  iPhone, iPad and iPod
25th May 2012 - A neew updated version is available! Get help from a special team of experts in qPCR while on the move. MIQE - qPCR helps you in reviewing scientific works and checking your own experiments, when qPCR is involved. Check your project's compliance to MIQE in minutes, have all required references to hand, and follow qPCR events and workshop news.
Over 4,000 downloads => http://www.***.com/

MIQE qPCR  -  Best Of The Web  -  Feb 2012 (Vol. 32, No. 3)
Rated  * * * => VERY GOOD => Nice user interface, strong reference resource
rated by Genetic Engineering News
http://www.***.com/

Android Market APP -  MIQE qPCR
Now MIQE qPCR app is even more interactive. For the first time ever, checklists are optimized in real time: you can reach 100% for every project if you are MIQE compliant.
Checklists are specific for each project type: just click on the kind of nucleic acid you are working with, the checklists adapts instantly by removing unnecessary items (Reverse transcription items are not relevant if working only on DNA for instance).
Moreover, some items may not apply to your specific experiments. You can now remove them and have the most accurate MIQE compliancy.
References have been updated, so you can keep in touch with latest MIQE related literature, symposium updates and more.
https://market.android.com/details?id=com.biorad.miqeqPCR

Since May 2012 a html5 version of the MIQE_qPCR APP is online
=> http://www.***.com/
To run this application you need a html5 compatible web browser!

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Follow our new established "GeneQuan Daily NEWS"
A daily updated newsletter around Gene Quantification and qPCR  -   http://www.***.com/

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GenEx 5 -  A Powerful Tool For qPCR Data Analysis
Download a free trail version here => http://www.***.com/

GenEx is a popular software for qPCR data processing and analysis. Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to advanced cutting-edge data analysis. View our webpage => http://www.***.com/

Basic data editing and management
Arguably the most important part of qPCR experiments is to pre-process the raw data into shape for subsequent statistical analyses. The pre-processing steps need to be performed consistently in correct order and with confidence. GenEx Standards streamlined and user-friendly interface ensures mistake-free data handling. Intuitive and powerful presentation tools allow professional illustrations of even the most complex experimental designs.

Advanced cutting-edge data analysis
When you need more advanced analyses GenEx Enterprise is the product for you. Powerful enough to demonstrate feasibility it often proves sufficient for most users demands. Current features include parametric and non-parametric statistical tests, Principal Component Analysis, and Artificial Neural Networks. New features are continuously added to GenEx with close attention to customers needs.

New features
Sample handling and samples individual biology often contribute to confounding experimental variability. By using the new nested ANOVA feature in GenEx version 5 user will be able to evaluate variance contributions from each step in the experimental procedure. With a good knowledge of the variance contributions, an appropriate distribution of experimental replicates can be selected to minimize confounding variance and maximize the power of the experimental design! For experiments with complex features, such as for example multifactorial diseases, analytical relationships and classifications may not readily be available. The support vector machine feature in the new version of GenEx is so easy to use that it will make this advanced supervised classification method easily available to novice users, while providing access to advanced parameters for experts.

Download a free trail version here => http://www.***.com/

GenEx PDF user guides:     NEW ! ! !
* GenEx user guide
* GenEx user guide - Exiqon Wizard
* GenEx user guide - Roche Wizard

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Please forward this qPCR NEWS   http://www.***.com/ ://q...  to further scientists and friends who are interested in qPCR !

Best regards,
Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages

If this newsletter is not displayed correctly by your email client, please use following
LINK http://www.***.com/

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